Basic setup for Multiple Site Directed Mutagenesis

Those are the objects of the MSD vignette.

Format

The following objects are included:

cuf

The Codon Usage Table which is being used to select the codon for an exchanged amino acid.

input_sequence

The sequence which should be modified. This is just an object of type character containing the sequence.

mutations

The desired mutations.

recognition_site_bsai

The BsaI recognition site.

Source

https://github.com/ipb-halle/GoldenMutagenesis/tree/master/vignettes