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MSD_BsaI_result_lv2
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Designed primers for Multiple Site Directed Mutagenesis |
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MSD_BsaI_setup_lv2
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Basic setup for Multiple Site Directed Mutagenesis |
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Point_Mutagenesis_BbsI_result
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Designed primers for Point Mutagenesis |
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Point_Mutagenesis_BbsI_setup
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Basic setup for Point Mutagenesis |
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base_distribution()
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Create a graphical evaluation of sequencing results |
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calculate_tm_nnb()
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Calculate melting temperature based on next neighbor calculation |
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cuf
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The Codon Usage Table which is being used to select the codon for an exchanged amino acid |
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domesticate()
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Domestication of the input sequence |
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get_cu_table()
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Select a Codon Usage Table |
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input_sequence
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The sequence which should be modified.
This is an object of type character containing the sequence |
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list_cu_table()
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List the available Codon Usage Tables |
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mutate_msd()
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Calculate Primers for Multiple Site Saturation Mutagenesis |
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mutate_spm()
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Calculate Primers for Point Mutagenesis |
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mutations
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The desired mutations |
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order_replacements()
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Order the replacement list |
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primer_add_level()
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Add a level to exisiting Primerset |
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primer_prepare_level()
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Prepare a Single Point Mutation Primerset to be used in Level 2 |
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primers
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Generated Primers
From Multiple Site Directed Mutagenesis or
Single Point Mutation |
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print_primer()
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A function to generate an output text for primers |
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recognition_site_bbsi
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The BbsI recognition site |
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recognition_site_bsai
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The BsaI recognition site |