Calculate melting temperature based on next neighbor calculation

The implementation is based on the explanations of http://biotools.nubic.northwestern.edu/OligoCalc.html.

calculate_tm_nnb(
  oligo_sequence,
  primer_concentration = 50,
  salt_concentration = 50,
  offset = 0
)

Arguments

oligo_sequence

A string containing an oligo sequence.
primer_concentration

The concentration of the primer in nanomole [default: 50]
salt_concentration

The concentration of Na+ in nanomole [default: 50]
offset

You can skip a prefix of your oligo sequence with this parameter. The first n bases are not considered in the calculation. [default: 0]

Value

An array or a list with values for the codons/amino acids.

The melting temperature in print('\u00B0')C

Details

More details at https://doi.org/10.1093/nar/gkm234

Examples

if (FALSE) {
GoldenMutagenesis::calculate_tm_nnb("AAAAAATGGTGTGTGATGTGTCCCTCTATC")
}