print_primer-methods.Rd
An example is shown in the vignette at https://github.com/ipb-halle/GoldenMutagenesis/blob/master/vignettes/Point_Mutagenesis.md
print_primer(primer) # S4 method for Primer print_primer(primer) # S4 method for Primerset print_primer(primer) # S4 method for Extended_Primerset print_primer(primer)
primer | An object of class Primer, Primer_MSD or Primerset |
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Textual output
#Load results of the Point Mutation vignette and print it data(Point_Mutagenesis_BbsI_result) print_primer(primers)#> Fragment 1 #> Start 1, Stop 66, Length 66 #> Forward #> TTGAAGACAACTCAATGGTGAGCAAGGGCGAGGAGG #> Temperature of binding site: 60.67248 °C #> Temperature difference: 0.6724812 K #> Reverse #> TTGAAGACTTCACGATGTCCCAGGCGAAGGGCAGGG #> Temperature of binding site: 61.41454 °C #> Temperature difference: 0.7420589 K #> #> Fragment 2 #> Start 67, Stop 143, Length 77 #> Forward #> TTGAAGACAACGTGTCCCCTCAGTTCATGTACGGCTCC #> Temperature of binding site: 59.45263 °C #> Temperature difference: 0.5473745 K #> Reverse #> TTGAAGACTTTTTCTGCATTACGGGGCCGTCGGA #> Temperature of binding site: 58.9345 °C #> Temperature difference: 0.5181218 K #> #> Fragment 3 #> Start 144, Stop 246, Length 103 #> Forward #> TTGAAGACAAGAAAAAGACGATGGGCTGGGAGGCC #> Temperature of binding site: 59.2929 °C #> Temperature difference: 0.7071014 K #> Reverse #> TTGAAGACTTCTCGTCACTCGAGTGCGGCCGC #> Temperature of binding site: 59.15354 °C #> Temperature difference: 0.1393632 K #> #> Input Sequence: #> ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACGATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGGTCGACAAGCTTGCGGCCGCACTCGAGTGA #> #> Modified Sequence: #> ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCGTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAAAAGACGATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGGTCGACAAGCTTGCGGCCGCACTCGAGTGA